Molecular Medicine

The Molecular Medicine Core (MMC) at the WVU Health Sciences Center offers a broad range of laboratory services in gene-based molecular medicine, as well as training and consultation. The Core provides treatment guidance for clinical trials conducted at the MBR Cancer Center such as EGFR mutation screening and detection of HPV subtypes. Other services include phenotype/genotype correlation and investigation of the significance of single nucleotide polymorphism (SNP) in disease susceptibility. The Core also helps WVU clinicians and investigators to understand advanced molecular-genetics techniques and trains laboratory personnel at West Virginia University and at institutions elsewhere in the state.

The Molecular Medicine Core offers a full-service gene analysis program -- including project, assay and primer design -- and experiments relating to genotyping analysis and gene expression assays. MMC services include sample preparation (fresh & paraffin-imbedded tissues, blood, and all other types), DNA/RNA extraction, reverse transcription-polymerase chain reaction (RT-PCR), products purification, DNA sequencing, SNP, and pathogen detection for clinical research. The following techniques are utilized: RT-PCR, Real-Time Quantitative PCR with SYBR green dye or with TaqMan probe chemistry, CEQ SNP Primer Extension, TaqMan Allele Discrimination, and direct DNA sequencing. The Molecular Medicine Core houses state-of-the-art specialized equipment, including ABI 7500 Fast Real Time PCR System, CEQ 8000 Genetic Analysis System, ABI Prism DNA Sequencer, ABI Thermal Cyclers, and other equipment required for gene amplification, DNA sequencing, SNP, and pathogen detection.

The MMC staff routinely use two different DNA sequencing systems to double-check findings, which enables the Core to provide high quality and highly reliable services. In addition, the Core is continually developing novel techniques and assays for more effective detection of DNA variation and gene expression. The Core provides timely reporting of all results and methodologies, with full interpretation of data and follow-up consultation as needed.

At present, the MMC offers a broad range of laboratory services in gene-based molecular medicine, as well as training and consultation. The Core provides EGFR mutation screening and detection of HPV subtypes for clinical research conducted at the WVU Cancer Institute. Other services include phenotype/genotype correlation and investigation of the significance of single nucleotide polymorphism (SNP) in disease susceptibility.

The Molecular Medicine Core houses state-of-the-art specialized equipment, including ABI 7500 Fast Real Time PCR System, CEQ 8000 Genetic Analysis System, ABI Thermal Cyclers, and other equipment needed for gene expression, DNA sequencing, SNP, and HPV detection. The MMC staff applies a range of strategies to provide high quality and highly reliable services.

The Scientific Director of the Molecular Medicine Core (Jing Jie Yu, MD) has extensive professional experience in molecular genetics. A special assistant assigned to the Core has expertise in genetic analysis and a variety of molecular testing techniques. With 18 years of experience in genomics investigation and genetic analysis, the MMC staff enable clinicians and investigators to reach their research and treatment goals.

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