The Animal Models and Imaging Facility (AMIF) is a full-service shared resource that provides state-of-the-art in vivo imaging to investigators at West Virginia University. The AMIF currently performs acute and longitudinal bioluminescence and fluorescence studies on mice using a Caliper IVIS Lumina II optical imaging system. In addition, the facility has a VisualSonics Vevo 2100 system for real time, micro-ultrasound imaging and image-guided injections. The AMIF offers services such as imaging-related procedures, assistance with experimental design and training on data analysis. The AMIF is growing rapidly to support translational research projects at West Virginia University by providing preclinical imaging in small animal models of human disease.